FastQCFastQC Report
Wed 15 Jun 2016
RPI01.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI01.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54642562
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2034900.3724020114576619No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1250530.22885639952240894No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1075970.19691060605833235No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1067430.19534772179972087No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT900980.1648861193587519No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT839380.1536128558540136No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA825810.15112944374753146No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG748850.13704518466758567No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT741440.13568909891157738No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT707110.12940645059797892No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT590970.10815195671096095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT439450.022.77842943
GACCTTG462650.021.65025544
TTATCCG272450.021.64755237
TATCCGA273200.021.55607438
CCCGCTA31700.020.356181
ATCCGAG296600.019.92239839
ATGGACC506000.019.81711841
ACTGCGT92950.019.6415546
CGCCGTT23850.019.4841751
GACTGCG94900.019.2844225
AATGGAC522250.019.17096140
TGGACCT532050.018.89218742
TCCGAGT317500.018.70142240
GGTTATC332700.017.85279535
CCCGTTT80300.017.7450281
GTCGTGA38550.017.7102721
CAATGGA573850.017.5656239
CCCGTAT30000.017.3252181
CCCGAAT57700.017.1761041
TACGTTC582150.017.1727827
Produced by FastQC (version 0.11.5)