FastQCFastQC Report
Wed 15 Jun 2016
RPI02.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI02.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98047815
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2977560.30368448292294936No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1816310.18524737139731265No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1719910.17541543378605634No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1496300.15260921418799595No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1418360.14466003143466277No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1397490.14253147813645822No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1363040.13901788632413686No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT1223890.12482583115187218No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1188670.12123370622792563No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1074480.10958734776496548No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1064890.1086092535565428No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT1059730.10808297971760003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT680150.021.82266243
TTATCCG411350.020.49317237
TATCCGA412800.020.38930538
ACTGCGT179800.020.2834856
GACCTTG733600.020.19641144
GACTGCG183550.019.9890845
ATGGACC755300.019.82344441
AATGGAC782450.019.051240
CCCGTAT59000.019.0008811
TGGACCT806250.018.5650742
CGCCGTT40650.018.4215581
GTTCGTT80400.018.3264371
ATCCGAG459700.018.31399539
CCCGATT70350.018.1894681
CCCGTTT157900.017.7982181
CCCGCTA54950.017.675791
CCTACGT852950.017.38386225
TACGTTC855450.017.35625327
TCCGAGT495000.017.25709740
GTCGTTT100000.017.1571621
Produced by FastQC (version 0.11.5)