FastQCFastQC Report
Wed 15 Jun 2016
RPI03.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI03.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68188258
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2187860.32085582828644776No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1466640.2150868849003299No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1262640.1851697105973876No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1168140.17131101955999523No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1041400.15272424176021626No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1023110.1500419617700162No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC983780.14427410654778716No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC938760.1376717968070104No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT858580.12591317408343236No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT837130.1227674711971671No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT804030.11791326301369952No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC801690.11757009542610694No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA769180.11280241240361354No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA769050.11278334753763618No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC758440.11122736116825276No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG757450.11108217488119436No Hit
CTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTC705370.10344449626503144No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT698630.10245605629051265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA43150.022.2032931
GGACCTT513100.021.22313343
GTACGTA220150.020.8191171
ATGGACC551750.019.8683441
GACCTTG549550.019.73912244
TACGTAT234450.019.5367282
AATGGAC567000.019.25623540
TGGACCT585650.018.71812242
ACGTATA249400.018.4096323
TTATCCG307250.018.1502237
GTCGTTT74800.017.9612731
TATCCGA310800.017.9572638
CGCCGTT29750.017.6197151
CGCTTAT326550.017.56946438
CGTTGAT404800.017.432551
CAATGGA632400.017.37242739
CTCGTTT550700.017.3533551
CTCCGTT363500.017.1289331
CCTACGT640600.017.0067725
TACGTTC641400.017.0061727
Produced by FastQC (version 0.11.5)