FastQCFastQC Report
Wed 15 Jun 2016
RPI04.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI04.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75718073
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT2006640.26501466829458276No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1870530.24703877500950136No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1526160.20155821979251903No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1341160.17712547967246867No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1220800.16122967101922944No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1026200.13552906979024676No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1004940.13272128570942368No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT957620.12647178699331135No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT941170.12429925415560958No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT871550.11510461973853983No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC827270.10925661037358941No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG822860.10867418667667361No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT808470.10677371570193023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA406000.022.90933838
TTATCCG406750.022.87230937
ATCCGAG439700.021.17862339
TCCGAGT468100.020.01144240
GGACCTT471250.019.41720643
GGTTATC498650.018.80219835
GTTCGTT58200.018.2409131
GTCGTTT84500.018.0894471
ATGGACC508950.018.02493741
GACCTTG508850.017.87828344
CCCGTAT43700.017.690831
GCCTATA536700.017.51637810
CCCGTTT112000.017.3646161
TATTGCC560300.017.014436
AATGGAC541500.016.85607140
TGGTTAT567000.016.70278234
CCTATAG567450.016.6654311
TGGACCT553750.016.53881342
GAGTTGT574550.016.40176843
GTCGTGA54700.016.3881381
Produced by FastQC (version 0.11.5)