FastQCFastQC Report
Wed 15 Jun 2016
RPI05.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI05.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68772211
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1817250.26424190433545897No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1696870.2467377412077096No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1341280.19503226383109887No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1060510.15420618074937276No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1034140.1503717831610794No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT923820.13433042017509078No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT902430.13122015227923964No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT889040.12927314493349648No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG812460.11813783331758812No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT811950.11806367545751874No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT791690.11511771811437035No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC789360.1147789184791514No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT772910.11238696397299194No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA769270.11185768042269281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA355200.022.78528438
TTATCCG357600.022.68144437
ATCCGAG387850.020.94101539
GGACCTT440650.020.83543443
TCCGAGT412850.019.81182540
GACCTTG476350.019.17655644
ATGGACC481450.019.05886341
GGTTATC431400.018.93360135
GTTCGTT55450.018.7478641
AATGGAC505250.018.16541740
TGGACCT520100.017.67204342
GTCGTTT72450.017.6623741
CCCGCTA31950.017.5784531
GTCGTGA48250.017.4372251
CGCCGTT28000.017.3838371
GCCTATA477750.017.23324810
CCCGTTT102150.017.0549131
CGCGTAA5950.016.6573891
CCCGTAT39950.016.5943811
TATTGCC504750.016.5337926
Produced by FastQC (version 0.11.5)