FastQCFastQC Report
Wed 15 Jun 2016
RPI07.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI07.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81789729
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG3751220.45864194023677474No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT2249160.27499296396984024No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1848560.22601370888513397No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1741120.21287758515497712No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1700230.20787817991180776No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1477990.18070606396067165No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT1422120.17387513290330134No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1416300.1731635521130043No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1292840.15806874723842157No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1245280.15225383617544447No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1177930.14401930589597625No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT1125900.1376578714425133No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA1054500.12892816896361156No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC1040660.1272360249536956No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA1040500.12721646259519945No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC975850.11931204711535356No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA963100.11775317167269256No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG929980.11370376346399191No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA914520.11181355057430255No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT867850.1061074551304602No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA866040.10588615594997264No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA841300.10286132626750726No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA841050.10283076008235703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT744450.024.60958543
GACCTTG796250.022.91152644
TTATCCG473150.021.94982337
TATCCGA479700.021.627538
ACTGCGT156100.021.5171626
ATGGACC865800.021.29069741
CGCCGTT33750.020.9471281
GACTGCG161500.020.9204775
AATGGAC883550.020.79304540
TGGACCT899450.020.4891742
GTCGTGA66600.020.4034771
ATCCGAG519250.019.9972839
CCCGCTA40800.019.4867881
CAATGGA957050.019.21432539
TCCGAGT551300.019.01852440
GTTCGTT66000.018.7202721
GGTTATC565250.018.47042835
GTCGTTT99300.018.0094341
CCCGTTT130950.017.7771741
TACGTTC1045900.017.6005627
Produced by FastQC (version 0.11.5)