FastQCFastQC Report
Wed 15 Jun 2016
RPI08.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI08.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54864221
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1662990.303110108863115No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1077760.19644132010914728No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT881500.16066937321501384No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA849870.15490423166675418No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC843780.15379421864023185No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT722570.13170149631760925No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT685980.12503230475103255No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT652690.11896459807567486No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG623650.11367153103294769No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT580820.10586498621752052No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC560630.10218499229215339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT377850.021.4933343
GACCTTG407200.019.79245644
ATGGACC418200.019.58487741
TTATCCG215050.019.18035737
TATCCGA218100.018.90216438
AATGGAC434600.018.81027240
GTCGTTT60500.018.31181
TGGACCT447700.018.24507942
GTCGTGA40700.018.2370221
GTACGTA218750.018.2040841
CGCGTAA3400.018.1383421
CCCGTAT29000.017.9998461
TACGTAT226750.017.5315972
ATCCGAG240600.017.18036339
CCCGCTA29250.017.1683041
CGTTGAT297100.017.0952361
CCCGTTT87750.017.0679051
CAATGGA481050.016.98907739
CGCTTAT235150.016.81127538
CCCGTCT68400.016.7764511
Produced by FastQC (version 0.11.5)