FastQCFastQC Report
Wed 15 Jun 2016
RPI09.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI09.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58645193
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2369430.4040279993621983No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1744010.2973832825479831No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1547940.26395002229765024No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1217830.20766066879513895No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1095850.18686101007460237No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1036980.1768226766684867No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1023790.17457355797260315No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1016260.1732895652675233No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT950660.16210365272393254No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT924720.15768044279434804No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG866950.14782967804368893No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA825480.1407583397329769No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA792720.1351722041395618No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA792230.13508865082940388No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT678470.11569064151600626No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA677090.11545532811188804No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA658000.11220015935491934No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC645850.11012837829692196No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC643010.10964411013192506No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG624870.106550932486487No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC607150.10352937196404147No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA601460.10255913046445254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT490900.023.9330843
GACCTTG516400.022.59321644
TTATCCG377200.022.13852737
TATCCGA379650.022.00743738
CGCCGTT22250.021.281731
GTTCGTT49050.020.8342321
GTCGTGA46850.020.6843381
ATCCGAG405500.020.65886939
ATGGACC574450.020.53456141
AATGGAC589000.019.92251440
ACTGCGT111300.019.8619636
TCCGAGT426750.019.69744740
TGGACCT598850.019.6390642
GACTGCG115000.019.376145
CCCGTAT32250.019.2582971
GGTTATC436900.019.19361935
CCCGCTA33950.018.6831971
CAATGGA649000.018.14144739
GCCTATA472000.017.92769410
CCCGTTT88850.017.896921
Produced by FastQC (version 0.11.5)