FastQCFastQC Report
Wed 15 Jun 2016
RPI10.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI10.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76419923
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG3238340.4237559883435109No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1932400.25286599673752613No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1884670.24662024325776932No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1538850.20136764597368148No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1361420.17814987853363842No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1358920.1778227387117362No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1328760.17387612390030804No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1326340.1735594525527067No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1161170.15194597879927202No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1143710.14966123428310704No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT1064930.13935240421532485No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1063630.1391822915079357No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA930080.12170648222192006No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA922350.12069496589259844No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT888170.11622231024755154No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG830710.10870332858095133No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA817040.1069145280347901No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA812370.10630343084747677No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA777620.10175618732303617No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA776790.10164757690216462No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC770730.10085458997387368No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC765610.10018460761861799No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC764650.10005898592700756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT666500.023.92302543
GACCTTG706650.022.52294344
TTATCCG422500.021.3789637
TATCCGA424750.021.22448538
ACTGCGT148250.020.8612926
ATGGACC776050.020.77039341
AATGGAC793100.020.30436540
GTCGTGA65000.020.1274321
TGGACCT807800.019.97298642
GACTGCG156900.019.8236355
CCCGCTA45750.019.4975131
ATCCGAG463450.019.49501439
GTTCGTT56000.019.2718471
CGCCGTT31500.018.9484651
CAATGGA865850.018.63881939
TCCGAGT488300.018.61572340
CCCGTTT119200.018.3295021
GGTTATC495450.018.32416235
CCCGTAT38550.017.9399221
GCCTATA531900.017.2487510
Produced by FastQC (version 0.11.5)