FastQCFastQC Report
Wed 15 Jun 2016
RPI11.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI11.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79975240
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG3375320.4220456231203557No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT2479310.31000969800153144No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA2223330.27800229170928403No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1814660.2269027263938189No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1621780.20278526203860095No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1506900.18842081624262708No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1462550.18287534992080048No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1389930.1737950395647453No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT1158940.14491235037244027No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA1141690.14275543280645359No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1059210.13244224087355036No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA1042300.13032783646538604No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA982240.1228180121747681No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT963890.12052355203935618No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA960990.12016093981087146No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA953930.1192781665925604No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA952050.11904309383754272No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC945660.11824409654788157No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC913340.11420284578076915No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG909810.11376145917161361No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC892650.11161579508857991No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG865480.10821849362377656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT700100.023.95528243
GACCTTG742300.022.57834444
TATCCGA525450.021.82938238
TTATCCG526850.021.78376437
ATGGACC820250.020.76433241
ATCCGAG562650.020.4135639
AATGGAC843400.020.05873340
CGCCGTT35100.020.0159051
TGGACCT851600.019.91457242
TCCGAGT590950.019.54798740
GTCGTGA61200.019.3967531
GTTCGTT61200.019.3247811
ACTGCGT125600.019.2117486
GGTTATC615750.018.78127535
GACTGCG129250.018.6864055
CAATGGA915600.018.5391539
CCCGTTT115250.017.561691
GTCGTTT85450.017.5520061
GCCTATA670650.017.36994410
CGTTGAT602500.017.26811
Produced by FastQC (version 0.11.5)