FastQCFastQC Report
Wed 15 Jun 2016
RPI12.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI12.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83329721
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2850940.342127630548529No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1812490.21750822854669105No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1575280.18904179458371162No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1444060.17329471197917487No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1334470.16014334189358442No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1204880.14459186776828403No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT1117840.13414661498746647No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1097510.13170690923110134No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1043410.12521462780368606No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1036440.12437819154584712No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT971460.11658025352082961No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA918400.11021277750347923No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA881420.1057749851340556No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT874980.10500215163326901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT620750.022.68616543
TTATCCG386450.021.60317237
TATCCGA389000.021.3938138
GACCTTG670250.020.92830844
CGCCGTT35200.020.3975181
ATGGACC700150.020.15028841
AATGGAC724700.019.45550740
ATCCGAG430600.019.42929839
TGGACCT739300.019.09500542
CCCGTAT51000.019.0013681
CCCGCTA40850.018.9781111
GTCGTGA63950.018.7008651
TCCGAGT457150.018.43107240
ACTGCGT116750.018.0301866
GTTCGTT71450.017.8167291
GGTTATC473700.017.716835
CCCGTTT135050.017.5966191
TGCCGAA145150.017.52109744
GTCGTTT92750.017.4294831
CAATGGA817050.017.32894939
Produced by FastQC (version 0.11.5)