FastQCFastQC Report
Wed 15 Jun 2016
RPI13.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI13.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67785957
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1897410.2799119587557051No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1383830.2041470034862826No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1299100.19164736436486396No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1298420.19154704860182176No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT928590.13698855059315604No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT925130.13647812038708845No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA923880.13629371641090796No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT910900.1343788655222497No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT889950.13128825488146462No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG832130.12275846455925966No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA771970.11388346999364485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT442650.020.91111443
GTCGTGA58500.020.8583281
TTATCCG299350.020.26776537
TATCCGA300650.020.18038238
GACCTTG478900.019.34657144
ATGGACC503400.018.5382841
ATCCGAG332750.018.2799939
GTTCGTT50000.017.8846991
GTCGTTT71250.017.7440431
AATGGAC527500.017.5909840
TGGACCT536800.017.33548542
GTCGGGT150150.017.294741
TCCGAGT354450.017.22320440
TCGTGAG67100.016.9508912
CCCGTTT102600.016.787461
GGTTATC367750.016.64757735
CGCCGTT28150.016.4310931
CGTTGAT386500.016.3837721
GCCTATA379550.016.27318410
TGCCGAA110950.015.95050244
Produced by FastQC (version 0.11.5)