FastQCFastQC Report
Wed 15 Jun 2016
RPI14.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI14.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61785698
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2070000.3350289900423234No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1366350.22114341089098No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1251360.20253230771949846No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1188550.19236652469314178No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1106140.17902848649536984No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT886570.14349113608783703No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT876690.14189206052183792No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT842840.1364134463610009No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT785180.12708118956590891No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG767410.12420511944366153No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT742690.12020419353359091No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA668420.10818361233047816No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA646780.10468118366162991No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA637000.10309829307099518No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT627220.10151540248036041No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC625930.10130661629816012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT454200.022.55744443
GACCTTG489350.020.83789644
ATGGACC505250.020.4296641
TTATCCG292300.019.99656137
TATCCGA294250.019.80439438
AATGGAC521750.019.7033340
TGGACCT538000.019.26768942
CGCCGTT24750.019.13121
CCCGCTA29300.018.7158991
CCCGTTT91900.018.5003851
GTACGTA299800.018.4236161
ATCCGAG323250.018.05509439
GTCGTGA50050.017.8649141
GTCGTTT68700.017.759551
CAATGGA585000.017.6592939
TACGTAT314450.017.5326272
CCCGTAT33950.017.449841
ACTGCGT86350.017.4493966
GTTCGTT49100.017.2686421
TACGTTC596800.017.18630227
Produced by FastQC (version 0.11.5)