FastQCFastQC Report
Wed 15 Jun 2016
RPI15.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI15.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55633501
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1623900.29189246961107124No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1525420.27419090522453365No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1331120.23926590562761813No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1075480.19331517532933978No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT917800.16497254055609406No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT916610.1647586406614964No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT901690.16207680332754898No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT803330.14439680867828183No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA790500.14209064426845974No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT745030.1339175113211013No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA723240.1300008065284261No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA709410.12751489430801777No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG706930.12706911973776377No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC696940.12527343911000677No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA628620.11299306869075165No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT581430.10451076950918475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG322800.022.27104837
TATCCGA325000.022.14107538
GGACCTT371750.022.06532343
TGCCGAA107000.020.47315444
ATCCGAG354500.020.37312739
GACCTTG404300.020.34323744
GTCGTGA49200.019.9657341
GTTCGTT46450.019.9623621
CGCCGTT21300.019.8535041
CCCGTTT92550.019.6570851
ATGGACC432750.019.1703141
TCCGAGT378700.019.1354240
GGTTATC378450.019.12382935
AATGGAC448100.018.400740
CCCGTAT32150.018.359861
CCCGAAT56600.018.2892951
TGGACCT461850.017.95754642
GCCTATA409250.017.81180610
ATGCCGA125250.017.68370443
CCCGCTA32950.017.5798781
Produced by FastQC (version 0.11.5)