FastQCFastQC Report
Wed 15 Jun 2016
RPI20.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI20.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46493503
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2495840.5368147889394352No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT1017170.21877680414831296No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT834570.1795024995212772No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG780920.16796325284416622No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA737870.15870389460652168No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA677550.14573003888306715No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT596350.12826523310149376No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG591830.12729305425749485No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC572740.12318710422830476No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA528560.11368470127966052No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT521200.11210168440093661No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA516810.11115746645289343No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC489930.10537601350451051No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG487050.10475657211718375No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT475090.10218416968925743No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA465000.10001397399546341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT490250.025.26387843
GACCTTG526950.023.54599244
ATGGACC578100.021.5695941
AATGGAC580800.021.34799240
TGGACCT596750.020.79208242
GTCGTGA43750.019.8344081
CAATGGA628950.019.71335639
TTATCCG127950.019.70354537
TATCCGA132050.019.00857738
GTCGTTT58200.018.7319641
CCCGCTA28250.018.476981
TCCTACA690050.017.9325334
ACTGCGT84750.017.7267156
CTCCTAC714000.017.36480933
TACGTTC716900.017.28316727
TCTCCTA721300.017.27446432
CCCGTTT79700.017.271211
CCTACGT716950.017.26658425
TACAATG721600.017.21251937
CTACGTT724900.017.09544626
Produced by FastQC (version 0.11.5)