FastQCFastQC Report
Wed 15 Jun 2016
RPI21.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI21.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37470197
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1129170.3013514980986089No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA885740.2363851996828306No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT709480.18934514809196226No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC638310.17035138619634158No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA617960.16492040327410074No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT583490.15572109215225102No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT507190.13535824217844383No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC491490.13116824552590423No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT466510.12450161390931572No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA461610.12319390794769507No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT436900.11659933359837954No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA429440.11460841799150402No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA427880.11419208711392684No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT419060.11183821638300968No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG403000.1075521433741061No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA402040.10729593975713553No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG400700.10693832220844743No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT377830.10083480479165882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT253700.021.72560943
GACCTTG274000.020.15603644
ATGGACC279450.019.73641
TTATCCG172350.019.4138837
AATGGAC284450.019.37343640
TATCCGA171950.019.33113138
TGGACCT296750.018.4815442
CCCGTAT19150.018.4006081
CGCCGTT20050.017.9041711
ATCCGAG187350.017.82458539
CCTACGT308900.017.73056425
TACGTTC311650.017.59530627
CCCGTTT57350.017.4726561
CAATGGA316150.017.45857839
CCCGCTA17400.017.3401281
GTTCGTT29750.017.3224721
CTACGTT317200.017.27355226
GTCGTGA27450.017.2495231
GTACGTA168000.017.2122021
CCCGAAT36800.017.0560321
Produced by FastQC (version 0.11.5)