FastQCFastQC Report
Wed 15 Jun 2016
RPI25.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRPI25.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72970809
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG11545721.5822381796534557No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3374530.46244930626985375No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2851640.3907918850125398No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2674290.3664876457653087No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG2544510.3487024516885923No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2130800.2920071778291508No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC1930490.2645564749049171No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG1882610.2579949469931188No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA1834930.25146082730150354No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1787360.24494178213098883No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG1655600.2268852466744613No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1522550.2086519281977537No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG1501810.2058096957647818No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1417020.19418998081822006No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1367570.18741329837798562No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA1339330.18354325768815308No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT1312080.17980888768822612No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA1302650.17851659010660004No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG1240790.17003922760401355No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT1235540.1693197618242111No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC1193380.16354210900964522No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT1173520.16082047274547825No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1138760.15605692407768154No Hit
CGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCT1127130.15446313607404297No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1124850.15415068236395735No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT1115430.15285975519334039No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1070260.1466696086650211No Hit
GCCGAAGATAATTAGTTTGGGTTAATCGTATGACCGCGGTGGCTGGCACG1066550.14616118618062737No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1044360.14312024415132907No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1026910.14072887694036668No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG995020.13635863623219527No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATTCGGGGAAGGTCG990320.13571454305789593No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG983830.1348251463129592No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC973040.13334647283408904No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG970310.13297235062859178No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG940580.12889811869839624No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT927000.12703710054797393No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC901550.12354940452969353No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT900760.12344114205997087No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG890980.1221008800930246No Hit
GTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGAC864750.11850629201603069No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC805990.11045375692627993No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA798700.1094547273006114No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT753760.1032961002255025No Hit
GGGGAGAGTAGTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTC744890.10208054566038867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT1749500.030.79815743
GACCTTG1783350.030.26761644
GTCGTGA86650.026.9693491
ATGGACC2083750.026.03754641
AATGGAC2084550.025.99673540
TGGACCT2106050.025.71149442
CAATGGA2154100.025.15183339
TCCTACA2182950.024.8492534
CTCCTAC2227250.024.3676933
TCGTGAG96350.024.271582
TCTCCTA2245450.024.21328732
TGGAGTC2255650.023.7986552
CCTACAA2281900.023.77818335
GGAGTCT2287400.023.7058123
GAGTCTT2296700.023.6174874
CCCGCTA51300.023.5710331
TACAATG2311350.023.46309937
CTACAAT2323600.023.368836
TACGTTC2361800.023.01749627
AGTCTTG2375800.022.9906395
Produced by FastQC (version 0.11.5)