FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI01.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI01.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11250431
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG421720.37484786138415493No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT258080.22939565604197742No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA221880.19721911098339256No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC217470.19329926115719478No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT185800.16514922850511238No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT176000.15643845111356178No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA168640.14989647952154012No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT153790.13669698520883333No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG151320.13450151376422823No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT143950.12795065362384783No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT118310.10516041563207668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA551.3561475E-732.0516051
GGACCTT93450.023.08022943
GACCTTG99000.021.69648644
ACTGCGT19200.021.4119436
TATCCGA57450.021.02060138
TTATCCG57750.020.98599837
ATGGACC105950.020.28575741
GACTGCG20100.020.01575
ATCCGAG61550.019.58558739
AATGGAC110350.019.53609840
CCCGCTA5700.019.3293691
TGGACCT111850.019.25503542
CAATGGA120450.018.0440639
TCCGAGT67950.017.9027540
GTCGTGA8250.017.8954811
CCCCGAT7650.017.8588221
TACGTTC121300.017.77896927
CGCCGTT5100.017.7147981
CCTACGT121750.017.71325525
GGTTATC69700.017.48209635
Produced by FastQC (version 0.11.5)