FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI01.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI01.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10911795
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG414370.37974503736552967No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT246700.22608562569219823No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA214160.19626468422473112No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC212750.1949725045237745No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT177500.16266801199985886No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT170040.15583137329834368No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA165880.15201898496076952No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG157040.14391765974342444No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT152170.13945459935785084No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT139750.12807242071538183No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT121540.11138405734345265No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA109700.10053341361343392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT88400.022.94506643
TATCCGA54150.021.97867438
TTATCCG55100.021.59973137
GACCTTG94100.021.22789444
CCCGCTA6500.020.3255711
ATCCGAG59350.020.12712939
CCCGATT8200.019.8711381
TCCGAGT61200.019.77051240
ATGGACC103250.019.6874141
AATGGAC106250.019.007340
TGGACCT106300.018.95696842
GGTTATC65600.018.24306335
ACTGCGT18950.018.2260746
CCCGAAT12400.017.9351311
CAATGGA116050.017.45891239
GACTGCG19900.017.245915
CCTACGT118000.016.98380925
GCCTATA71450.016.90319610
TACGTTC119100.016.8638927
CTACGTT120550.016.69754426
Produced by FastQC (version 0.11.5)