FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI01.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI01.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10973470
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG403730.3679146158872262No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT244950.2232201846817825No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA213620.19466950745753167No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC208450.18995814450670573No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT176840.16115230642631728No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT165720.15101877528256785No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA161760.147410071745765No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG148560.13538105995642216No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT146280.13330332155644478No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT140220.1277809115986101No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT116330.1060102228374434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT85800.022.82070443
TTATCCG53250.021.81406637
TATCCGA53800.021.67284438
GACCTTG91200.021.54135544
ATCCGAG58750.020.03420839
ATGGACC102300.019.31198941
ACTGCGT16600.019.084326
AATGGAC103500.019.02431140
CGCCGTT5450.018.9780831
TCCGAGT62950.018.80237840
CCCGATT8000.018.4304431
TGGACCT105950.018.3975142
GTCGTGA8150.018.0912321
CCCGCTA6100.018.0381151
GTCGTTT12700.017.8477921
GGTTATC66000.017.83291235
GACTGCG18100.017.6242945
CCCGAAT12250.017.6052021
TGCCGAA21000.017.28546544
GTCCGGT6250.017.2530961
Produced by FastQC (version 0.11.5)