FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI01.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI01.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10731421
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG403840.3763154944717946No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT241970.22547806110672577No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC210550.1961995526967025No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA210210.19588272606209373No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT177910.1657841957742595No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT165310.1540429734328753No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA162550.15147108663428635No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG150570.14030760697954167No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT147930.13784754134610877No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT137250.12789545764722118No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT116240.10831743531448444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT88300.022.92115643
CGCGTAA602.833994E-422.046581
GACCTTG93450.021.44610644
CGCCGTT4400.021.0444641
ATGGACC98550.020.6934341
TTATCCG53300.020.10079237
CCCGCTA5600.020.0781351
TATCCGA53250.020.03591738
AATGGAC102000.019.79948640
ACTGCGT18450.019.792976
TGGACCT103700.019.53845842
GACTGCG19150.019.1843455
TGCCGAA21700.018.55242744
GTCGTTT11800.018.4967081
ATCCGAG57900.018.4648139
CCCGTAT5600.018.1096921
GTTCGTT8050.017.8015861
CAATGGA112650.017.78993239
CCTACGT114250.017.69557425
TACGTTC114300.017.61084627
Produced by FastQC (version 0.11.5)