FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI01.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI01.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10775445
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG391240.36308477283304774No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT258830.24020353683768977No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC218210.2025067178200065No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA216100.20054856203154486No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT182930.16976561060819298No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA166980.15496343770489293No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT162310.15062950996455368No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT145940.1354375619753987No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG141360.13118715746774262No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT141270.13110363423505944No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT118550.11001865816214551No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC110850.10287278158813858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG54650.023.14717537
GGACCTT87050.023.1238743
TATCCGA55350.022.8146938
CGCGTAA602.8627706E-422.013671
GACCTTG91700.021.90344
CCCGTAT5500.021.6134221
ATCCGAG60400.020.9071739
GTCGCGT956.835762E-720.8550571
ATGGACC98550.020.47062141
ACTGCGT17000.020.187286
TCCGAGT64150.020.16512740
AATGGAC100850.019.93841440
TGGACCT103450.019.41549542
GGTTATC66100.019.27069735
GACTGCG18450.018.7199825
CCCGCTA5000.018.4914821
CGCCGTT5250.018.4495531
CAATGGA111500.018.11290439
CCTACGT114350.017.48758725
GCCTATA73000.017.41859810
Produced by FastQC (version 0.11.5)