FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI02.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI02.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19894044
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG610900.30707683163865523No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT365220.183582583812522No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA347920.17488651377266484No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT305260.15344290984779163No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC289590.14556618051111178No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA279220.1403535651172783No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG276310.1388908157637532No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT246880.12409744343583437No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT242270.12178016696856608No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT219430.11029934386392229No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT216020.10858526300635506No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT214630.10788656142511799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT141050.022.5605743
GACCTTG152600.020.92555244
GACTGCG34850.020.502765
TATCCGA80850.020.34982738
ACTGCGT34150.020.3436156
ATGGACC157800.020.29617941
TTATCCG81400.020.29181537
CCCGTAT12350.019.8067841
AATGGAC164050.019.49557740
CCCGCTA9950.019.2687761
TGGACCT167550.019.19388442
CCCGTTT32500.019.189381
GTTCGTT17000.018.4076081
ATCCGAG90500.018.1559939
CCCGATT15100.017.8049531
CCTACGT178750.017.75982525
TACGTTC180200.017.65356427
CAATGGA184600.017.4444539
CTACGTT183100.017.32593226
CCCGTCT26600.017.3150021
Produced by FastQC (version 0.11.5)