FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI02.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI02.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19478135
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG603820.30999887822935823No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT358370.1839857871402986No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA340480.17480112957426366No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT304520.1563394031307412No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG284240.14592772870708617No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC281010.14426945906268746No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA280140.14382280439066678No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT244500.12552536472305997No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT241680.1240775875103032No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT211690.10868083622995732No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT210630.1081366362847367No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT210240.10793641177658948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT136750.022.08820343
TTATCCG79350.021.09779537
GACTGCG36250.020.8163975
TATCCGA80250.020.80646738
ACTGCGT36350.020.6371336
GACCTTG149200.020.15658444
ATGGACC151550.019.91625241
CCCGTAT11900.019.6150231
CGCCGTT7800.018.9151631
AATGGAC159000.018.9000540
ATCCGAG88700.018.87394139
CCCGCTA10200.018.782311
CCCGTTT30650.018.6798251
TGGACCT163600.018.48965542
CTCCGTT111750.018.069731
CGCGTAA1102.7758706E-618.0168861
TCCGAGT95800.017.6821340
CCCGAAT18900.017.5932061
CCTACGT172700.017.41303325
CAATGGA175400.017.33325239
Produced by FastQC (version 0.11.5)