FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI02.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI02.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19363494
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG584250.3017275704477715No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT352830.18221401571431273No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA332340.17163224777511746No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT292530.1510729416912051No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC273970.141487894695038No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA269630.1392465636625291No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG265410.13706720491663335No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT234540.12112483418540063No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT234270.12098539654052105No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT209590.1082397629270833No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT208710.10778529949192021No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT203580.10513598423920807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG79450.022.23530637
TATCCGA79500.022.19364738
GACTGCG36750.020.8922985
GGACCTT133350.020.82044643
ACTGCGT36800.020.3856546
CCCGCTA10650.020.2497231
ATCCGAG88850.020.08108339
GACCTTG138900.019.90897444
CGCCGTT7550.019.2370991
TCCGAGT94550.018.96355640
GTCGTGA11850.018.7562351
ATGGACC148700.018.64160341
AATGGAC153700.018.12105840
GGTTATC98950.017.94206635
TGGACCT156250.017.83940142
CCCGTAT10150.017.344691
GTTCGTT17150.017.194251
TACTAAC64200.017.13359828
CTCGTTT139950.017.1079731
CTCCGTT113450.016.9337181
Produced by FastQC (version 0.11.5)