FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI02.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI02.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19871727
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG597230.30054257488541386No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT363200.18277223715885388No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA351290.17677879733351812No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT308060.1550242714183825No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC288280.1450704309695881No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA286130.14398849179037132No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG280540.14117544992440767No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT252810.12722095065013725No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT236870.11919950389817655No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT220520.1109717338608768No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT218460.10993508515892957No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT208730.1050386813385671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA78850.021.56587238
GGACCTT133550.021.3983243
TTATCCG79550.021.37707337
GACTGCG39200.020.708115
ACTGCGT39800.020.3959276
GACCTTG144200.019.66531844
CCCGTAT11800.019.6164231
ATGGACC146850.019.49026541
ATCCGAG88200.019.32957839
AATGGAC151600.018.90865740
GTTCGTT14750.018.8317661
CCCGCTA10100.018.7710931
TGGACCT156250.018.4303742
CTCCGTT115500.018.1132641
TCCGAGT95700.018.0226940
GGTTATC99350.017.44886835
GAACGAT49350.017.07359344
CAATGGA170700.016.79191239
CCCGTTT30150.016.6709381
TACGTTC170700.016.63785427
Produced by FastQC (version 0.11.5)