FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI02.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI02.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19440415
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG581360.2990471139633593No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT376690.19376643965676657No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA347880.17894679717485454No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT285930.14708019350409957No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC285510.14686414873345038No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA282370.1452489568766922No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG256540.13196220348176724No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT245160.12610841898179642No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT233580.12015175601961171No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT217780.11202435750471376No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT217720.11199349396604959No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT211370.10872710279075833No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA202310.10406670845246872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT132200.020.90073643
TTATCCG84050.020.65240537
TATCCGA85350.020.41516938
CGCCGTT8100.019.8403131
GACCTTG140350.019.62394744
ATGGACC144150.019.55060441
ACTGCGT34700.019.2091756
CCCGCTA10500.019.0793151
GACTGCG35250.018.7846435
AATGGAC150400.018.60675440
ATCCGAG94200.018.52053839
CCCGTAT10850.018.4638521
TGGACCT154400.018.10929942
CCCGTTT31500.017.751451
GTTCGTT15950.017.5288621
CTCCGTT112050.017.230521
TCCGAGT101950.017.19896940
TACTAAC65050.017.04485528
CCCGATT14600.017.0386921
TGCCGAA33750.017.01222844
Produced by FastQC (version 0.11.5)