FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI03.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI03.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13949151
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG453010.32475811610326677No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA298090.21369759349511666No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA256780.18408288791195968No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT240060.17209649533509244No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG212450.15230317601408142No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT209230.14999479179772301No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC202760.14535651667976066No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC191780.13748506987988016No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT172660.12377814248336692No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT170010.12187838528667444No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT167190.11985675687359036No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC161950.11610025585069657No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA156120.11192079001797314No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA155380.11139029178191562No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC154270.11059454442782933No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG152650.10943318342456827No Hit
CTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTC142520.10217109270664573No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT140640.10082334043125636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA7850.024.423021
GGACCTT101000.021.00788543
GTACGTA44450.019.6819671
ATGGACC108950.019.44410541
GACCTTG109350.019.36305444
AATGGAC113800.018.730440
TTATCCG62350.018.48468637
TATCCGA62650.018.36277838
TGGACCT117500.018.29134442
TACGTAT47800.018.213222
CTCGTTT110850.017.8720251
CGTTGAT83350.017.6876541
GTCGTTT14300.017.5678481
ACGTATA50100.017.464853
CTCCGTT76000.017.078571
CAATGGA125200.017.07762539
ACTGCGT20300.016.8946086
CGCTTAT66550.016.89003838
ATCCGAG68700.016.84203539
TACGTTC128950.016.41751127
Produced by FastQC (version 0.11.5)