FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI03.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI03.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13647870
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG434250.31818151843474474No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA291960.21392349135799213No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA246540.18064357295314212No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT227460.16666336944885907No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG206880.15158409334203798No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT201880.14792051800024472No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC192510.14105497780972415No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC185640.13602122529010022No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT167060.12240737931999644No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT162090.11876578543025394No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT161340.11821624912898496No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC159750.11705123217029471No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC151920.11131407318504646No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA150520.11028827208934433No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA148880.10908661937723614No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG147670.10820003414452217No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT137000.10038196436513536No Hit
CTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTC136640.10011818694052625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT100900.021.25952543
GTACGTA43700.020.7976251
CCCGCTA8500.019.935031
TACGTAT46050.019.7333972
GACCTTG109050.019.60913344
ATGGACC109900.019.57858341
GTTCGTT10800.019.3573071
AATGGAC111750.019.21508840
CGCCGTT5550.018.6358831
ACGTATA49350.018.547393
TGGACCT116450.018.49623342
TTATCCG62000.018.13278437
CTCGTTT108400.017.9054161
TATCCGA62300.017.86889838
CGTTGAT85450.017.589511
ACTGCGT20950.017.536866
CGCTTAT64300.017.41574738
CGTTTAG53100.017.2789843
TAGCCGT53300.017.2118767
CCCGATT8250.017.0714781
Produced by FastQC (version 0.11.5)