FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI03.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI03.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13264383
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG418400.3154311813825038No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA289740.21843458531014973No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA248830.18759259288577537No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT235780.17775421593299892No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT196110.1478470577937926No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC195520.1474022576097207No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG195330.14725901687247722No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC182210.13736786701650577No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT171520.12930869080001683No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT166650.12563720453488111No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA154790.11669596693641912No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT153540.1157535936650804No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA152390.11488661025544873No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC151610.11429856933413338No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG151240.11401962684581711No Hit
CTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTC140520.10593783367081604No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC140490.10591521671230392No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT139620.10525932491545216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA45200.021.2362791
GGACCTT95850.021.0925743
CGCCGTT4650.020.3585031
TACGTAT48000.019.941852
GACCTTG104450.019.33468244
ATGGACC104950.019.2640441
ACGTATA50700.018.9230463
TATCCGA59900.018.87804838
AATGGAC106750.018.85706340
CCCGCTA7850.018.7903771
TTATCCG60450.018.7062937
TGGACCT110650.018.3707442
CCCGTAT6300.017.8221441
GTCGTTT13050.017.7136941
CGTTGAT79250.017.6124741
ATCCGAG65800.017.25220539
CTCGTTT111550.017.2098581
CCCGTCT16900.017.195611
CGCTTAT65500.017.12969838
CTCCGTT68650.017.092951
Produced by FastQC (version 0.11.5)