FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI04.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI04.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15477991
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT411030.2655577199909213No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG384600.2484818604688425No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA308270.19916667479649006No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC274130.1771095486487878No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA245320.15849602186743744No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT209690.13547623848598955No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT203650.13157392325657769No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT198090.12798172579374156No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT190620.12315551805140604No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT175920.1136581614500228No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC168540.10889010078892021No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG164270.10613134482375652No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT162870.10522683467124383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG84450.022.84205837
TATCCGA84900.022.64531538
ATCCGAG92850.020.75416439
GGACCTT94700.019.9413343
TCCGAGT97850.019.76109740
GGTTATC102650.018.76918235
ATGGACC102350.018.76403641
GACCTTG103000.018.20490544
GTCGTTT16550.018.1111471
CCCGTAT8700.017.9863971
TATTGCC112150.017.6818566
GCCTATA109550.017.6189610
GTCCGTA6300.017.49181
CCCGTTT23450.017.2933861
CCCGCTA7950.017.188171
AATGGAC110350.017.1040140
TGGACCT111200.017.09262742
TGCCTAT117650.016.798059
CCTATAG116250.016.68631211
TGGTTAT117250.016.67794234
Produced by FastQC (version 0.11.5)