FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI04.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI04.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15033149
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT389940.25938677252517084No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG377760.2512846776147832No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA305560.2032574811837493No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC267650.1780398770743242No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA244420.16258735944145836No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT200790.13356483062863278No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT196820.13092400002155238No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT193090.12844281660482443No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT192670.12816343402170763No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT176130.11716108181991677No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG171940.11437390795501329No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC166000.11042263999378973No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT160930.10705009309759385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG81700.023.1028337
TATCCGA82350.022.86704838
ATCCGAG89700.021.04237739
GGACCTT96950.020.17310343
TCCGAGT95250.019.9552640
GGTTATC98300.019.4028535
CCCGCTA7050.019.0532231
GACCTTG104000.018.6363744
ATGGACC106000.018.49185641
GTCGTTT16500.018.4171681
GCCTATA105750.017.91110
AATGGAC110150.017.65535440
GTTCGTT12150.017.3989351
TGGTTAT110850.017.32509434
CCCGTAT9700.017.2531971
GTACGTA73400.017.1004121
TATTGCC113650.017.0724646
TGGACCT114400.017.01873842
CCTATAG112850.016.84260611
TATAGTC113750.016.70934313
Produced by FastQC (version 0.11.5)