FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI04.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI04.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15067862
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT392830.2607071925665366No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG368860.2447991626151076No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA299640.1988603293552861No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC263120.17462331417688853No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA237370.1575339620179691No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT199400.13233463380537996No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT193710.1285583847263799No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT188910.12537279675112503No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT184100.12218057213425502No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT166560.11053990274134445No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG165110.10957758970715288No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC161330.10706893917663965No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT155870.10344533285478724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA80050.022.48088838
TTATCCG80900.022.32626537
ATCCGAG88050.020.53881839
GGACCTT91500.019.78831743
TCCGAGT93900.019.37639440
GGTTATC97500.018.86328535
GTCGTTT17550.018.180691
GACCTTG100950.018.08817344
CCCGCTA7250.017.9074381
GCCTATA104450.017.5867310
ATGGACC103050.017.57041241
AATGGAC106400.017.07935340
CCCGATT10700.017.0692251
CCCGTAT8550.016.9862421
TATTGCC109550.016.9692046
CCCGTTT23200.016.6933751
CCTATAG110350.016.64643311
TGGTTAT111100.016.47498334
TATAGTC112450.016.37474413
TGGACCT111800.016.25430342
Produced by FastQC (version 0.11.5)