FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI04.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI04.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15161296
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT401320.26470032641009056No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG375780.24785480080330866No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA304800.20103822258994217No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC267130.17619206168127052No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA248020.1635875983161334No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT205850.13577335341253147No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT205370.1354567577864056No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT191960.12661186748151343No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT191680.1264271866996067No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT179320.11827484932686494No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG168160.11091400101943792No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT166210.10962783128830146No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC164250.10833506581495408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA80100.022.57504538
TTATCCG81600.022.1611637
ATCCGAG87200.020.83784739
CGCCGTT6100.019.876431
GGACCTT96600.019.6313243
GTTCGTT12900.019.481421
TCCGAGT95050.019.18771640
GTCGTGA11300.018.533211
GTCGTTT17150.018.252811
GGTTATC100250.018.1920235
GACCTTG104900.017.9311744
GCCTATA102900.017.8515610
ATGGACC106400.017.67843841
CCCGTTT23000.017.3482721
CCCGGTT13400.017.1093711
TATTGCC108500.017.0923396
CCCGATT9950.017.0597691
TCGTGAG12450.016.9626082
AATGGAC111450.016.81823240
TGGTTAT109750.016.79771834
Produced by FastQC (version 0.11.5)