FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI04.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI04.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14977775
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT411520.2747537601546291No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG363530.2427129530253993No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA307890.20556457818334167No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC269130.17968623510501391No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA245670.16402302745234187No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT210470.14052153941423207No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT205390.13712984739055034No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT186270.12436426638803161No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT181400.1211127821054863No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT173620.1159184191243359No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC167150.11159868538551287No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT162590.10855417443512137No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG153380.10240506350242275No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA151560.10118992974590686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG84750.023.67471537
TATCCGA85550.023.37617938
ATCCGAG92300.021.66665339
TCCGAGT98100.020.72204440
GGACCTT94650.020.104743
GTCGTTT17500.019.8765141
GGTTATC101800.019.83921635
GACCTTG101050.018.67878744
ATGGACC102100.018.63857741
GCCTATA110500.018.33602710
TATTGCC113100.018.1085976
CCCGTTT22950.017.8423251
AATGGAC107900.017.63698440
TGGTTAT115300.017.57274634
CCCGTAT9200.017.4685231
CCTATAG116200.017.37978711
TGCCTAT117850.017.3791289
TTGCCTA121000.017.1630528
TATAGTC118250.017.13435713
TGGACCT111600.017.05116342
Produced by FastQC (version 0.11.5)