FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI05.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI05.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13668224
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG369620.2704228435237819No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT331620.24262113351376158No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA264160.19326578200649916No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC213830.15644314872217488No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA209740.15345080677635953No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT183990.1346114901248326No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT182300.13337504565333433No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT175260.12822441306200424No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG170330.12461750700017793No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT164240.12016191715909835No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT160290.11727200256595151No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC156530.11452109652285478No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT154670.11316027598025902No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA153760.1124944981879138No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA137570.10064950647574988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA67850.022.33957738
TTATCCG68350.022.20826137
CGCGTAA1004.587855E-822.0203191
GGACCTT90250.020.93909543
ATCCGAG74000.020.48297539
CCCGCTA6600.019.6848321
ATGGACC97950.019.29282841
TCCGAGT79250.019.26492740
GGTTATC81200.018.82923935
GACCTTG99650.018.7872944
AATGGAC100850.018.69442440
TGGACCT103450.018.18211242
CCCGTAT8350.017.6689991
GTCGTTT15150.017.4418351
GCCTATA91650.016.82608810
TACTAAC49650.016.79264628
CCCCGTT11150.016.7867891
GTTCGTT11650.016.6333731
CAATGGA114250.016.59803439
CCTACGT113900.016.4942825
Produced by FastQC (version 0.11.5)