FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI05.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI05.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13679303
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG356810.2608393132310908No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT331270.24216877131824627No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA262700.1920419483361104No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC207910.1519887380226902No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA201240.14711275859596062No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT179070.13090579249542172No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT177130.1294875915826998No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT173450.12679739603691798No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG159000.11623399233133443No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT158710.11602199322582445No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC155780.11388007122877532No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT151450.11071470527409182No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT151420.1106927743321425No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA147730.10799526847237759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA68200.022.77446738
TTATCCG68400.022.7400437
ATCCGAG73750.021.18022739
GGACCTT84700.020.4680843
TCCGAGT78900.020.02081140
GTCGTGA10500.019.0724031
GGTTATC83250.018.92092735
GTTCGTT11850.018.7566971
GACCTTG92750.018.5721944
ATGGACC94300.018.3843741
AATGGAC98200.017.76625840
GTCGTTT13300.017.539111
CGCCGTT5400.017.5237921
CGTTGAT77500.017.264551
GCCTATA92100.017.17420210
TGGACCT102800.016.86426242
CCCGTTT19350.016.8319381
TCGTGAG12300.016.8157312
CCCGAAT13350.016.8140471
TATTGCC95750.016.7955936
Produced by FastQC (version 0.11.5)