FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI05.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI05.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13862559
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG365780.26386181656647956No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT338190.24395928630493113No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA273130.19702711454645566No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC212150.15303812232647665No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA210920.15215084025972406No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT188980.1363240365649661No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT183400.13229880572555183No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT179510.12949268601850494No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG166450.12007162602518048No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT163220.11774160889053745No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT163020.11759733538374842No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC158170.11409870284411414No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT155300.11202837802169138No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA155110.11189131819024178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG72150.022.99029537
TATCCGA72800.022.66300238
CCCGTAT8000.021.4940071
ATCCGAG78550.021.03203639
GGACCTT92500.020.69126543
TCCGAGT82450.020.25165740
ATGGACC97550.019.55245641
GGTTATC88150.019.06692135
GACCTTG99350.019.04320744
AATGGAC101050.018.72283640
GTTCGTT10750.018.6614631
GTCGTGA10150.018.4614411
TGGACCT105750.018.16115242
ACTGCGT16900.017.9636276
GTCGTTT16200.017.1462151
CCCGCTA7350.017.0962281
CCCGTTT20200.016.9158211
TGGTTAT100550.016.86870834
GCCTATA100350.016.79241210
CCTACGT113150.016.70109625
Produced by FastQC (version 0.11.5)