FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI06.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI06.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805855
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC492830.3118021771046236No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG442840.2801746567964846No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC404920.2561835471728673No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG397500.2514890842665582No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTCAGCG311450.1970472334460869No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT301460.19072679079999152No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA288670.1826348527175531No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA264100.1670899802636428No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA243770.15422765804190913No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT242470.15340517801789275No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC237250.15010260438299605No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT237120.1500203563805944No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC228490.1445603543750085No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA227060.14365562634859044No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC225730.1428141660163275No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA222800.1409604225775828No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT219680.1389864705199434No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT219620.13894850990345034No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA219300.13874605328215397No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA216310.13685434922691622No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG215480.13632922736542882No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC215300.13621534551594963No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC213890.13532327102836259No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT207590.13133740629659071No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA204870.12961652501557178No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA204570.12942672193310645No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC202680.12823096251357485No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT197890.12520043996354516No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC196010.12401100731342912No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCG195660.12378957038388623No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT189140.11966451672497314No Hit
CTACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGAC187900.11887999731744978No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT185470.1173425923494806No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC183080.11583049445917351No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC180190.11400205809809086No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT179620.11364143224140676No Hit
CTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTCAGCGCCCGTCGG178730.11307834976342628No Hit
AGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA176630.11174972818616899No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA171920.10876981979146334No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG170330.10776386345439713No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT161600.1022405937546561No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC159310.10079176355850411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG34750.026.6563033
CGCTCCG41050.024.70663843
GCTCCGC43200.023.73128344
GTTACGA40700.023.405064
GGACCTT95150.023.35277443
TTACGAC41050.023.3126895
TACGACT42000.023.152046
CTACGAA43150.022.6432821
GACCTTG98200.022.58229344
GCAGACG103100.022.3440572
ATGGACC105250.021.4462441
CAGACGT107050.021.437313
CCCGCTA6300.021.3072051
TGGACCT108700.020.68460742
GTCGTTT16250.020.4484671
AATGGAC110350.020.39525840
GACTGCG23800.020.3358355
GCGCTCC50350.020.27423142
TTATCCG49350.019.88236837
ACGACTT49400.019.683927
Produced by FastQC (version 0.11.5)