FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI08.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI08.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11210488
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG342080.305142826967033No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA221490.1975739147127226No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT179130.15978787007309583No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA173270.1545606221602485No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC170800.15235732824476508No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT148130.1321351934010366No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT141170.12592672147724523No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT131300.11712246603359283No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG126080.1124661120907493No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT117520.10483040524194842No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC116000.10347453206318939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT77850.022.06334943
GTCGTGA8400.022.0384221
GACCTTG82750.020.54215444
ATGGACC85150.020.48622741
AATGGAC88400.019.73226240
TTATCCG44600.019.3805137
CCCGTAT6400.019.2836191
CGCGTAA808.877285E-519.2836191
TGGACCT90600.019.13248842
TATCCGA45450.018.97144538
GTCGCGA1051.7656603E-618.8900761
GTACGTA44600.018.2829971
GTCGTTT13150.018.0999971
TACGTAT46100.017.8357032
CAATGGA98300.017.61072339
ATCCGAG50100.017.34264639
CCCGTCT13450.017.2047161
CCTACGT101050.017.12175825
TACGTTC100800.017.12060427
GACTACC101900.017.02170419
Produced by FastQC (version 0.11.5)