FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI08.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI08.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10958027
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG343190.31318594122828863No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA215770.19690588460860703No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT173190.15804852461122793No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA170920.1559769838128707No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC170210.15532905695523472No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT150210.137077596176757No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT140860.12854503826281866No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT131020.11956531955980762No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG130000.11863449506010525No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT117740.10744634960289841No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC111010.10130473305094065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA801.3008139E-724.7716031
GGACCTT80000.022.0548143
GACCTTG84150.020.99328444
ATGGACC83950.020.85928541
AATGGAC90200.019.41393540
TTATCCG41950.019.29820637
TATCCGA42600.019.00375238
TGGACCT92600.018.9346142
GTACGTA42450.018.3623031
CAATGGA98950.017.74141339
GTCGTTT11700.017.690641
CCCGAAT10350.017.6579111
GTCGTGA8150.017.5613271
TACGTAT44300.017.5365662
TACGTTC100200.017.49805327
CCTACGT101150.017.3554625
CTACGTT101650.017.2484526
CGCTTAT50800.017.19205938
ATCCGAG48200.016.9784139
GACTACC103600.016.90286619
Produced by FastQC (version 0.11.5)