FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI08.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI08.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10924014
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG326280.2986814187532166No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA210270.19248419125057878No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT169420.155089511968769No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA166190.15213272337439332No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC162230.14850768224940028No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT142230.13019939373933428No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT134960.12354433086592528No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT126090.11542460491171101No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG122450.11209249640287901No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT112010.10253556980062456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT78300.020.67993743
CCCGTAT6100.020.5629561
ATGGACC81000.020.12641341
GACCTTG80650.020.02179544
GTACGTA41050.019.9420721
GTCGTGA8800.019.755361
AATGGAC83900.019.48318740
GTCGTTT12850.018.8377951
TTATCCG41500.018.81964537
TATCCGA41350.018.78150638
TACGTAT42950.018.7498072
TGGACCT87250.018.70990842
CGCTTAT46950.018.32197638
ACGTATA45550.017.824483
CAATGGA93550.017.6615739
CCCGCTA5650.017.5268781
CCCGTCT14000.017.2904051
CCCGTTT17500.017.2275311
CCTACGT96950.016.88248625
TACGTTC97050.016.8650927
Produced by FastQC (version 0.11.5)