FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI08.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI08.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11012364
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG330530.3001444558134838No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA215680.19585258896273314No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT180160.1635979341038854No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA172450.15659671256780106No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC171620.1558430142701422No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT146500.1330322898879841No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT137230.12461447878039629No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT132810.12060080832780318No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG126950.11527951673228382No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT118650.10774253375569495No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC115250.10465509494600796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT76600.021.051843
CCCGCTA5400.020.00281
ATGGACC82350.019.71545441
GACCTTG82400.019.64991644
CCCGTAT5200.019.5003761
TTATCCG44600.019.286737
TATCCGA44650.019.21469538
AATGGAC84400.019.08027540
GTCGTGA9250.018.8266851
GTACGTA45500.018.7978761
GTCGTTT13350.018.4937631
TGGACCT87550.018.46907842
TACGTAT47750.017.9213432
CCCGTCT12850.017.840981
GTTCGTT7900.017.3002781
ATCCGAG50250.017.16091339
ACGTATA49950.017.0879733
CGCTTAT43900.016.98734738
TACGTTC95500.016.8391527
CCTACGT96750.016.6215925
Produced by FastQC (version 0.11.5)