FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI08.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI08.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10759328
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG320910.2982621219466494No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA214550.1994083645372648No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT179600.1669249231922291No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC168920.15699865270396068No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA167040.15525133168168123No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT135500.12593723325471626No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT131760.12246117973167099No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT131470.12219164616972363No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG118170.10983027936317213No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT114900.1067910560956967No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC110420.10262722727664775No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT108640.10097284886193636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA651.8051776E-523.7091271
GGACCTT72650.022.0748543
GTTCGTT7700.020.871951
GACCTTG79500.020.14466344
ATGGACC80350.019.96023641
AATGGAC82700.019.47303240
CCCGTTT17050.019.2394561
TGGACCT84850.019.05642142
TTATCCG44300.018.9708937
TATCCGA44550.018.91381538
CGTTGAT58350.018.56331
GTCGTTT12000.018.1628861
CGCCGTT4400.017.5124261
TACGTTC92000.017.47943327
CCTACGT92700.017.39482125
GTACGTA40200.017.3605771
ATCCGAG48750.017.28431539
GTCGTGA7950.017.1694151
CCCGAAT9900.017.123261
CAATGGA94750.017.08939439
Produced by FastQC (version 0.11.5)