FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI10.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI10.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396865
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG649550.42187159528904095No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA385560.250414613624267No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT374400.2431663848452266No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC311360.2022229850037654No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA275820.179140363963703No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT272440.1769451118782947No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT272330.1768736687630891No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT271680.1764515049005106No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG237370.15416774778501985No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT227880.14800415539137352No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT221700.14399035128255006No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA217130.1410222145871903No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA189480.12306401335596565No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA188340.12232360288928948No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT179730.11673155541728787No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA171590.1114447648920738No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG167810.10898971966046335No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA167640.10887930757332742No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA159680.10370942396390433No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA158260.10278715829488666No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC158240.10277416863757655No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC154430.10029963892000092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT135350.023.90946643
GTCGTGA12450.022.6637331
GACCTTG143300.022.50618244
TTATCCG85250.021.13513437
ATGGACC157550.020.8895341
TATCCGA85850.020.85813938
GTTCGTT11500.020.3188341
AATGGAC162500.020.09074440
TGGACCT164350.019.9315242
ACTGCGT28800.019.7070476
CGCCGTT6650.019.5578541
GACTGCG29150.019.5458935
ATCCGAG92250.019.53030439
CCCGCTA8250.019.2384191
CAATGGA175850.018.57739
GTCGTTT15100.018.5403371
CCCGTTT24350.018.4680921
GGTTATC99950.018.07073635
TCCGAGT100200.018.04760640
TCGTGAG13650.017.7278372
Produced by FastQC (version 0.11.5)