FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI12.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI12.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16958783
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG587760.34658147344653206No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT368970.2175686781297927No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA321740.1897188023456636No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC294650.17374477873795544No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA268670.15842528322934493No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT245020.1444797070638854No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT228180.13454974923613328No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT224390.1323149190599349No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG211830.12490872723591073No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT210220.12395936665974203No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT198870.11726666943022974No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA185030.10910570646490375No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT179210.10567385643179701No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA178070.10500163838407509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT125850.022.9224143
CGCCGTT6400.022.0388831
TTATCCG79600.021.96769337
TATCCGA80650.021.71075438
GACCTTG137850.020.8785744
GTCGTGA12450.020.35721
CCCGCTA7700.020.0353491
ATGGACC145000.020.02604941
ATCCGAG89650.019.60539239
AATGGAC150500.019.23527740
TGGACCT152650.018.97921642
TCCGAGT94350.018.81527940
GTCGTTT18800.018.7564951
ACTGCGT22700.018.594656
CCCGTTT27100.018.1353171
GGTTATC97800.017.96906535
CAATGGA167050.017.36910239
TGCCGAA31350.017.34187744
CCCGTAT9050.017.2901741
TACGTTC167450.017.25118627
Produced by FastQC (version 0.11.5)