FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI12.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI12.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16497346
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG576290.3493228547185711No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT353670.21437993723354049No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA308440.18696340611392886No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC286840.17387039103138166No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA264730.16046823531494095No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT247720.15015748593743503No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT222450.13483987060706615No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT219790.13322749004597467No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG215020.13033611588191216No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT204300.12383810098909243No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT191750.11623081676289022No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA185160.11223623484650198No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT174860.1059928063580651No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA173910.1054169561576753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA858.185452E-925.901771
GGACCTT123300.022.82064443
TATCCGA77350.021.24546638
TTATCCG77450.021.21797237
GACCTTG131650.021.2061244
ATGGACC136350.020.87813641
AATGGAC142900.019.82878940
CGCCGTT5900.019.7775381
TGGACCT145800.019.61551542
ATCCGAG85050.019.37374139
CCCGCTA8900.019.2953641
CCCGTAT9850.019.222531
GTTCGTT14100.018.8935971
TGCCGAA29000.018.28266544
GTCGTGA14650.018.184281
TCCGAGT92100.018.03432740
CAATGGA159300.017.81475839
ACTGCGT24250.017.780766
CCCGTTT26700.017.5637281
CCTACGT160950.017.5225725
Produced by FastQC (version 0.11.5)