FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI12.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI12.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16535655
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG558960.33803317739756905No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT353000.21347808720005346No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA306060.18509094438653928No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC280790.16980881616119833No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA259570.15697594077767105No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT235470.1424013744844096No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT214100.129477786032667No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT213890.12935078773716552No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG203000.1227650189847333No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT202400.12240216671187201No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT188580.11404446936030051No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA176950.1070111828046727No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA172600.1043805038264284No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT170770.1032738043942015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT129050.023.33850743
GACCTTG136200.022.0642644
TATCCGA75600.021.15626138
TTATCCG75700.021.12831337
ATGGACC142500.021.05848341
AATGGAC146750.020.4637340
CCCGTAT9650.020.2944981
TGGACCT153050.019.77938342
ATCCGAG83450.019.2453939
GTCGTGA13100.018.4772341
CAATGGA164350.018.41954839
TACGTTC166100.018.14529827
GTCGTTT19150.017.9255051
TGCCGAA28500.017.90848744
ACTGCGT24150.017.8550056
CCTACGT168050.017.83001725
CCCGTTT25150.017.7612551
CTACGTT170150.017.72632226
TCCGAGT91400.017.66770240
GTCCGTA7000.017.6037651
Produced by FastQC (version 0.11.5)