FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI12.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI12.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16551043
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG555480.3356163113104111No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT374120.22604013535582018No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA321730.19438654107780398No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC292350.17663539391445No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA270460.16340964131384347No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT227860.1376710821185106No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT226480.1368372978065491No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT214100.129357406660112No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT213810.129182191116294No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT201210.12156937783316737No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG196050.1184517495362679No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA186140.11246421146993575No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA177660.10734066729208545No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT173770.10499036223880272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT119400.022.0728443
TTATCCG83300.021.57821737
TATCCGA83350.021.38050338
GACCTTG127550.020.55860344
CCCGCTA7750.019.8831651
ATGGACC133500.019.84162141
ATCCGAG91750.019.47100839
CCCGAAT17000.019.2941881
CGCCGTT6050.019.2845571
AATGGAC137700.019.09292440
TGGACCT138850.019.04426842
GTCGTTT19100.018.7863921
GTTCGTT13800.018.6636221
TCCGAGT99050.018.23589940
CCCGTTT26650.018.1724971
GGTTATC100800.017.94112835
CGCGTAA1002.4730276E-517.6108041
CAATGGA153000.017.3130539
CCCGGTT15900.017.3062131
TACGTTC152300.017.30469327
Produced by FastQC (version 0.11.5)