FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI13.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI13.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13871051
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG392950.283287834497905No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA281730.2031064553075322No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC266130.19186001118444448No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT264630.1907786223264553No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT189500.13661545905930272No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT188180.13566383686427222No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA187430.1351231424352776No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT184080.13270804065243508No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT182580.13162665179444585No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG168450.12143996875218754No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA156530.11284653196070003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGA12050.022.1326471
GGACCTT89650.020.99239343
TATCCGA60300.020.71961838
TTATCCG60850.020.49382637
GTTCGTT9850.019.2440831
GACCTTG97450.019.22097444
GTCGTTT15850.019.1904351
ATGGACC102700.018.46423741
ATCCGAG67650.018.43636739
TGCCGAA22700.017.79345744
TGGACCT107500.017.68071642
TCCGAGT71850.017.66481840
TCGTGAG14100.017.6191332
AATGGAC108150.017.45102140
GTCGGGT30350.017.4296051
GTCCGGT8350.017.157811
CCCGTAT7100.017.074161
CGCCGTT6000.016.8982451
GGTTATC75800.016.7708935
GCCTATA77050.016.57750910
Produced by FastQC (version 0.11.5)